Lentivirus-mediated cyclooxygenase 2 and aggrecanase 1 silencing and insulin-like growth factor 1 overexpression in human bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.10.003

Abstract

Abstract:

BACKGROUND: Cyclooxygenase 2, aggrecanase 1, and insulin-like growth factor 1 are involved in pathological injury of the articular cartilage.

OBJECTIVE: To observe the expression of shRNA vectors carrying cyclooxygenase 2, aggrecanase 1 and overexpression vectors carrying insulin-like growth factor 1 in bone marrow mesenchymal stem cells.

METHODS: Lentiviral vectors carrying the silencing gene cyclooxygenase 2, aggrecanase 1, the over-expressing gene insulin-like growth factor 1 and binding green fluorescent protein were constructed with recombinant lentiviral technology, and then the recombinant lentiviral vectors were used to transfect passage 3 human bone marrow mesenchymal stem cells cultured in vitro (experimental group). The human bone marrow mesenchymal stem cells transfected with no target gene lentivirals were used as negative control group. The human bone marrow mesenchymal stem cells transfected with no treatment served as blank group.

RESULTS AND CONCLUSION: Cyclooxygenase 2 and aggrecanase 1 transfected in human bone marrow mesenchymal stem cells were significantly inhibited at gene and protein levels, while the expression of insulin-like growth factor 1 was increased significantly at gene and protein levels. We confirmed that cyclooxygenase 2 and aggrecanase 1 were successfully silenced while insulin-like growth factor 1 overexpressed by using lentiviral vectors in human bone marrow mesenchymal stem cells, which brings a new hope for the systemic gene treatment of arthritis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Mesenchymal Stem Cells, Lentivirus Infections, Cyclooxygenase 2, Insulin-Like Growth Factor I

CLC Number:

R394.2

Yuan Yu-qi, Zhang Hai-ning, Kong Xia, Sui Ai-hua, Wang Ying-zhen. Lentivirus-mediated cyclooxygenase 2 and aggrecanase 1 silencing and insulin-like growth factor 1 overexpression in human bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1488-1494.

Figures/Tables 4

References

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